SAGM红细胞保存液细菌内毒素检查法可行性研究

Study on the Feasibility of Bacterial Endotoxin Test for SAGM Erythrocyte Preserve Fluid

目的 :研究SAGM红细胞保存液细菌内毒素检查法的可行性 ,以替代热原检查法。方法 :按《中国药典》(二部 )2000年版规定 ,用不同厂家生产的鲎试剂 (TAL)对SAGM红细胞保存液进行细菌内毒素检查法干扰试验。结果 :SAGM红细胞保存液经2倍稀释后对鲎试剂反应无干扰作用。结论 :选用灵敏度为0

OBJECTIVE:To study the feasibility of bacterial endotoxin test for SAGM erythrocyte preserve fluid as a substitute for pyrogen test.METHODS:Interference test was carried on endotoxin detection of SAGM erythrocyte preserve fluid with test agent of limulus(TAL).RESULTS:There was no interference action of twofold diluted SAGM fluid on limulus test.CONCLUSION:It is feasible to use 0.25EU/ml TAL in bacterial endotoxin test.