摘要
目的 :克隆大鼠脑组织中神经生长因子基因。方法 :采用 RT- PCR方法 ,大鼠脑组织中的 m RNA逆转录成 c DNA,再以 c DNA为模板 ,扩增 NGF基因片段 ,克隆入 T载体 ,并经序列测定。结果 :RT- PCR法扩增出一特异产物与预期长度 5 0 4bp相符 ,T载体克隆测序与大鼠 NGF基因 10 0 %同源。结论 :采用 RT- PCR和 T载体技术获得了大鼠脑组织中的 NGF基因克隆 ,为在分子水平对 NGF m RNA的研究奠定了基础。
Objective:To develop the gene cloning of NGF from rat brain.Methods:The gene frame of NGF was amplified by RT-PCR from rat brain. RT-PCR product was ligated into pGEM-T vector, and the DNA sequence was detected.Results: The product of RT-PCR was the 504bp which matched the size of purpose. The sequence of NGF was completely homogeneous with the NGF sequence reported. Conclusions:NGF gene had been cloned from rat brain using RT-PCR and T vector techniques. This study provides an approach for the study of NGF mRNA. [
出处
《南通医学院学报》
2001年第1期4-6,共3页
ACTA Academiae Medicinae Nantong
基金
江苏省教育厅青蓝工程资助
关键词
神经生长因子
逆转录聚合酶链
基因克隆
脑组织
Nerve growth factor gene
T-A clone
Reverse transcription polymerase chain reaction
Rath
