猪hnRNPK基因启动子的克隆及序列分析

Cloning and Sequence Analysis of the Promoter Region of Porcine hnRNPK Gene

首次克隆了猪hnRNPK基因启动子序列,并进一步对该序列进行了分析.结果显示:该基因启动子约1 kb,与已报道的人的相应序列相似度为78.9%,具有相同的"TCTCGCGAGA"核心启动子序列和转录起始位点.利用在线软件分析发现,猪hnRNPK基因启动子不含TATA盒,而含有CAAT盒的GC富集区,存在两处CpG岛,具有SP1、UCE.2、GCF、EARLY-SEQ1、TTR_inverted_repeat、NGFI-C、EARLY-SEQ1等多种转录因子潜在结合位点,并且具有8种基序结构.

The porcine hnRNPK gene promoter were cloned firstly and analysis by the bioinformatics software .The results showed that the length of porcine hnRNPK promoter is about 1 kb, and had 78.9 percent of the similarity com-paring with human hnRNPK promoter , which had the same core promoters ’ sequence of “TCTCGCGAGA” and tran-scriptional start site .The results of bioinformatics analysis showed that no TATA box and two CpG island are found in porcine hnRNPK gene promoter region , and exited many transcription factors-binding sites such as SP1, UCE.2、GCF, EARLY-SEQ1, TTR_inverted_repeat, NGFI-C, and 8 motifs.