HPLC法测定萘敏维滴眼液中苯扎溴铵含量

Determination of Benzalkonium Bromide in Moisten Eye Drops by HPLC

目的：建立萘敏维滴眼液中苯扎溴铵含量的测定方法。方法：采用十八烷基硅烷键合硅胶色谱柱，以0．005mol·L^-1的醋酸铵溶液一乙腈（35：65）为流动相，柱温为30℃，流速为1．0mL·min^-1，检测波长为214nm，采用外标法进行计算。结果：在49．5～495．0μg·mL^-1的浓度范围内线性良好，r=0．9999，苯扎溴铵的回收率为100．99％o，RSD〈2％。结论：本法测定萘敏维滴眼液中防腐剂苯扎溴铵具有简便、灵敏、重现性好的优点，可用于测定萘敏维滴眼液中苯扎溴铵的含量。

Objective： To develop a RP-HPLC method for the determination ot Benzalkonlum Bromide m Moisten Eye Drops. Methods： The sample was analyzed on an Eclipse XDB-C18 Column （4. 6mm× 250mm, 5μm） with mobile phase consisting of 0. 005mol · L ammonium aeetate-acetonitrile （35：65）, isocratic elution at the flow rate of 1. 0mL ·min^-1. Column temperature was set up at 30℃, and the wavelength was at 214nm. The external standard method was adopted to calculate. Results： The linear rages of the Benzalkoni- um Bromide were 100. 99%, RSD〈22%. Conclusion. The method is stable, accurate, which can be used for the quality control of Benzalkonium Bromide.