FISH技术检测HTERC基因在宁夏部分地区宫颈癌早期筛查中的临床意义研究

FISH Detection of HTERC Genes for the clinical significance of the early screening for cervical cancer in parts of Ningxia

目的评估人染色体末端酶基因(HTERC)检测在宫颈病变筛查中的临床意义。方法收集120例患者,根据细胞学TCT分为正常组23例,异常细胞组88例,宫颈癌9例。人乳头瘤病毒(HPV)阳性(77例),HPV阴性(43例);组织病理学诊断分为宫颈炎组28例,宫颈上皮内瘤变组78例,宫颈癌组14例。同时选取23例未见上皮内细胞病变(NILM)妇女为对照组,应用荧光免疫杂交技术进行检测,以HTERC基因在该组中的表达建立实验阈值。结果①对照组阈值是8.7%,即对照组每例病例中发生扩增的细胞不超过8.7%。②HTERC基因在所有细胞学异常组与NILN组中比较,HTERC基因阳性表达率差异有统计学意义(P<0.05)。③HTERC基因在HPV感染组与非感染组间中的表达差异有统计学意义。④组织病理学分级中,HTERC基因的阳性表达率随着病变级别的递增,差异有统计学意义(P<0.05),且HTERC基因的阳性表达在宫颈癌组中明显高于CIN组,差异有统计学意义(P<0.05)。结论①HTERC基因随着宫颈病变级别的递增显著扩增,提示HTERC基因可作为宫颈癌前期病变进程中预测指标;②HTERC基因扩增与高危型HPV感染有相关性。

Objective To detect the telomerase genes of the expression and correlation of cervical intraepithelial neoplasia and cervical carcinoma HTERC genes, and to assess the clinical significance of the detection of HTERC genes by the cervical lesion screen- ing. Methods The 120 cases of cervical lesion patients with cervical liquid - based cytological （TCT）, high risk HPV testing, colpos- copy and pathological diagnosis. TCT was divided into the following types according to cytology： No epithelial within tumor NILM （23 cases）, epithelial within tumor 88cases, cervical cancer Scc （9 cases） ;HPV positive （77 cases）, HPV negative （43 cases）. Organiza- tional pathological diagnosis was divided into： Cervical Inflammatory Group （28 persons）, CIN Group 78cases）, Cervical Cancer Group （ 14 cases）. At the same time, 23 NILM women was selected as the comparative group in order to establish the experimental threshold with the expression of HTERC genes in the group. Results The comparative threshold was 8.7% ;The expression rates of HTERC genes showed significant difference with the comparison of all abnormal cytology groups with the normal groups （ P 〈 0.05 ） ;The comparison be- tween the HPV groups and non - infected groups showed significant differences of the expansion of HTERC ; And as the disease levels in- crease, the rate of the positive expression of HTERC genes was significant （ P 〈 0.05 ）. And in CIN Group at different levels, the posi- tive expressions of HTERC genes were statistically different （P 〈 0.05 ）. Conclusion HTERC genes are increasing with the significant increase of the levels of cervical lesions. The positive expressions of HTERC genes in SCC Group, CIN Group, and the normal groups are statistically significant.Objective To detect the telomerase genes of the expression and correlation of cervical intraepithelial neoplasia and cervical carcinoma HTERC genes, and to assess the clinical significance of the detection of HTERC genes by the cervical lesion screen- ing. Methods The 120 cases of cervical lesion patients with cervical liquid - based cytological （TCT）, high risk HPV testing, colpos- copy and pathological diagnosis. TCT was divided into the following types according to cytology： No epithelial within tumor NILM （23 cases）, epithelial within tumor 88cases, cervical cancer Scc （9 cases） ;HPV positive （77 cases）, HPV negative （43 cases）. Organiza- tional pathological diagnosis was divided into： Cervical Inflammatory Group （28 persons）, CIN Group 78cases）, Cervical Cancer Group （ 14 cases）. At the same time, 23 NILM women was selected as the comparative group in order to establish the experimental threshold with the expression of HTERC genes in the group. Results The comparative threshold was 8.7% ;The expression rates of HTERC genes showed significant difference with the comparison of all abnormal cytology groups with the normal groups （ P 〈 0.05 ） ;The comparison be- tween the HPV groups and non - infected groups showed significant differences of the expansion of HTERC ; And as the disease levels in- crease, the rate of the positive expression of HTERC genes was significant （ P 〈 0.05 ）. And in CIN Group at different levels, the posi- tive expressions of HTERC genes were statistically different （P 〈 0.05 ）. Conclusion HTERC genes are increasing with the significant increase of the levels of cervical lesions. The positive expressions of HTERC genes in SCC Group, CIN Group, and the normal groups are statistically significant.