格列卫对K562细胞中miR-146a、miR-29b及3种甲基化酶表达的影响

Influence of Gleevec on expression of miR-146a,miR-29b and DNMT in K562 cells

目的观察BCR/ABL抑制剂格列卫作用后K562细胞中microRNA(miR)-146a及miR-29b的表达水平变化及DNMT1、DNMT3a、DNMT3b3种甲基化酶水平的改变。方法 MTT法检测格列卫作用于K562细胞时的IC50浓度。通过茎环引物法及荧光定量PCR的方法检测miRNAs以及甲基化酶基因的水平。结果格列卫作用于K562细胞时的IC50浓度为40.85μmol/L。格列卫作用后miR-29b表达出现了上升的趋势而3种甲基化酶基因表达水平均有所降低,miR-146a的水平显著升高(P<0.05)。结论格列卫能影响K562细胞中miR-146a、miR-29b和3种甲基化酶的表达水平。

Objective To investigate the changes of microRNA （miR）-146a ,miR-29b expression levels and the 3 kinds of meth-ylase DNMT1 ,DNMT3a and DNMT3b levels in K562 cell lines after BCR/ABL inhibitor Gleevec treatment .Methods The half maximal inhibitory concentration（IC50 ） of Gleevec on K562 cells was detected by the MTT method .The stem loop primers method and the fluorogenic quantitative PCR were adopted to detect miRNAs and the methylase gene level .Results IC50 of Gleevec acting on K562 cells was 40 .85μmol/L .After Gleevec action ,miR-29b showed the increasing trend ,but 3 kinds of methylase expression level were decreased to some extent .Gleevec could significantly increase the miR-146a level in K562 cells（P＆lt;0 .05） .Conclusion Gleevec can influence the expression of miR-146a ,miR-29b and DNMTs levels in K562 cells .