雪菊的组织培养研究

Research on Tissue Culture of Coreopsis tinctoria Nutt.

以雪菊带芽茎段、茎尖、叶片为外植体,以MS为培养基,添加不同浓度6-BA和NAA,进行组织培养实验。结果表明,适合带芽茎段愈伤组织诱导的培养基为MS+6-BA 1.0mg/L+NAA 0.3mg/L;适合茎尖愈伤组织诱导的培养基为MS+6-BA 1.0mg/L+NAA 0.1mg/L;适合叶片愈伤组织诱导的培养基为MS+6-BA 3.0mg/L+NAA 0.3mg/L;适合雪菊愈伤组织增殖和不定芽诱导的培养基为MS+6-BA 2.0mg/L+NAA 0.3mg/L;适合雪菊试管苗生根的培养基为1/2MS+6-BA 0.3mg/L+NAA 0.03mg/L,蔗糖减半。以雪菊无菌苗的带芽茎段、茎尖和叶片为外植体,成功地诱导出了愈伤组织。其中带芽茎段愈伤组织诱导率最高,时间最短,效果最好,茎尖次之,叶片最差。

The experiment of tis sure culture was designed to use Coreopsis tinctoria wi th bud stern section, stern tip and leaf as explant and to use MS as medium, adding 6-BA and NAA with different concentration. The result showed that the medium suitable for bud stern section callus induction was MS＋6-BA1. 0 mg/L＋NAA 0.3 mg!L;The medium suitable for stern tip callus induction was MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L; The medium suitable for leaf callus induction was MS＋6-BA 3.0 mg/L＋ NAA0.3 mg/L; The medium suitable for callus proliferation and adventitious bud induction was MS＋6-BA 2.0 mg/L＋NAA 0.3 mg!L; The medium suita hle for tube seedling rooting was 1/2MS ＋ 6-BA0. 3mg/L ＋ NAA 0.03 mg/L and sucrose in half was suitable for Coreopsis tinctoria tube seedling rooting culture. The bud stern section vstern tip and leaf with st erit ity seedling of Coreopsis tinctoria were used as explant s , the callus was successfully induced-among which the bud stern section callus had the highest callus induction rate with the shortest tim evhest effect, but stern tip s leaf were the worst.