浓缩血小板滤除白细胞对血小板功能的影响

Study of Platelet Function Following Concentration after Leukocytes Filtration

目的分析手工法制备的浓缩血小板滤除白细胞后血小板功能的变化。方法采用富血小板血浆法(PRP法),以400 ml新鲜全血制备浓缩血小板,将7袋ABO同型的浓缩血小板汇集,国产血小板型去白细胞滤器过滤,测定过滤前后的血小板计数、白细胞计数、pH值、血小板CD62p阳性表达率、血小板聚集和血小板的抗低渗休克反应(hypotonic shock response,HSR)等指标。结果血小板去白过滤后,血小板回收率为(87.46±3.56)%,白细胞去除率(98.61±1.03)%。CD62p+过滤前后分别为(9.63±3.86)%和(9.72±3.91)%(P>0.05),最大聚集过滤前后分别为(57.44±12.58)%和(59.28±15.23)%(P>0.05),HSR过滤前后分别为(75.83±8.67)%和(73.42±7.24)%(P>0.05)。结论去白细胞滤器过滤浓缩血小板未增加血小板的活化,对血小板聚集功能及抗低渗休克能力无明显影响,血小板回收率及剩余白细胞数符合国家相关标准。

Objective To evaluate the effect of platelet concentration after leukoeytes filtration.Methods Concentrated platelets were prepared from 400 ml fresh peripheral blood by platelet-rich plasma（PRP）method.Six bags of ABO-matched platelets were pooled and filtered.The number of platelets and leukocytes were counted,and pH,CD62p expression （%）,platelet aggregation and hypotonic shock response（HSR） were detected before and after filtration.Results The filtered leukocytes and platelet recovery was （87.46 ± 3.56）%,and the residual leukocytes was （98.61 ± 1.03）%.The CD62p expression （%） before and after filtration was （9.63 ± 3.86） % and （9.72 ± 3.91） % （P 〉 0.05）,respectively.Platelet aggregation was （57.44±12.58）% and （59.28±15.23）% （P〉0.05）.Hypotonic shock response was （75.83± 8.67） % and （73.42 ± 7.24）% （P〉0.05）.No significant effect of filtration was noted on the founctions of leukocytes and platlets.Conclusion Filtration with leukocyte filters will not activate platelet and have no influence on platelet aggregation and HSR.Besides,both platelet recovery and residual leukocytes met the requirements of Chinese standards.