基于复杂生物体系的噬菌体随机肽库筛选

Screening of Phage Display Peptide Libraries Using Complex Biological Systems as Selector Targets

近年来 ,噬菌体肽库生物淘筛方法又取得了新的进展 ,出现了以活细胞、病毒及组织器官等复杂生物体系 ,代替纯化的抗原、抗体或受体分子等作为筛选靶标的方法 ,并取得了较好的应用效果 .其中 ,以肿瘤组织筛选噬菌体肽库获得的短肽能选择性富集于肿瘤组织 ,在肿瘤靶向性治疗中具有潜在的应用前景 .

During recent years, some new methods for phage display panning have been developed bq using complex biological systems instead of purified antigens, antibodies or receptors as selector targets. These complex biological systems include living cells, viruses, mouse tissues and tumors etc. The peptides identified toy using tumors as selector molecules can selectively home to blood vessels of experimental tumors and may have potential applications in targeted anti-tumor treatment.