淫羊藿合女贞子对激素干预哮喘大鼠糖皮质激素受体亚型及转录因子的影响

Effects of combination of herba Epimedium,Fructus Ligustrilucidi and Dexamethasone on subtypes of glucocorticoid receptor and transcriptional factor of asthmatic rats

目的:研究地塞米松合用中药淫羊藿女贞子对哮喘大鼠糖皮质激素受体(GR)亚型及转录因子的影响。方法:实验大鼠随机分为7组:正常对照(N)组、哮喘模型(A)组、激素干预(GC)组、淫羊藿女贞子(YN)组、淫羊藿女贞子合激素干预Ⅰ组(YN+GC组Ⅰ)、淫羊藿女贞子合激素干预Ⅱ组(YN+GC组Ⅱ)、淫羊藿女贞子合激素干预Ⅲ组(YN+GC组Ⅲ)。哮喘大鼠在激素干预不同阶段合用淫羊藿女贞子,激素撤停2周后处死,检测血清GR亚型含量、肺组织GR亚型mRNA和54种转录因子蛋白表达。结果:中药合激素干预的3个组均显著抑制血清中GRβ含量,但上调肺GRβmRNA表达。GC组肺组织有43种转录因子表达较哮喘组下调,仅有1种上调;与GC组比较,中药合激素干预的3个组分别有49种、45种、46种转录因子表达上调。结论:淫羊藿女贞子合用地塞米松的协同抗炎及抑制激素的副作用与影响GR亚型及上调肺转录因子的表达有关。

Objective： To observe the effects of the compatibility of Epimedium and Fructus Ligustrilucidi on subtypes of glucocorticoid receptor （GR） and transcriptional factor （TF） of asthmatic rats with glucocorficoid （GC） intervention. Methods： Dexamethasone was injected into asthmatic model rats with the oral gavage of Epimedium and Fructus Ligustrilucidi at different treatment stages of GC withdraw. After GC withdraw 2 weeks, the rats were sacrificed. The contents of serum subtypes of GR and the expression of subtypes of GR mRNA and 54 TFs protein of lung tissue were observed. Results： The contents of serum GR Ⅰ in group I, I and III of Chinese medicine and GC significantly decreased, while the expression of GRI3 mRNA in lung tissue significantly increased. 43 TFs showed increased activities, only I showed decreased activities, compared with asthma group. Gl：oup I, II and III of Chinese medicine and GC had 49, 45 and 46 kinds of TF expression significantly increased respectively, compared with group GC. Conclusion： The mechanism of synergistic anti-inflammatory and inhibiting adverse effects of Epimedium and Fructus Ligustrilucidi combined Dexamethasone is related to GR subtypes and increasing the expression of TFs in lung tissues.