补肾、健脾、活血法对骨质疏松症小鼠骨骼、骨骼肌α-酮戊二酸脱氢酶的影响

Effects between invigorating kidney,invigorating spleen and promoting blood flow to α-KGDHC expressed in bone and skeletal muscle in osteoporosis mice

目的:观察中医不同治法对骨质疏松症小鼠骨骼、骨骼肌α-酮戊二酸脱氢酶(α-KGDHC)含量变化的影响。方法:除正常组外,将护骨素(OPG)基因敲除小鼠随机分为模型组、补肾组、健脾组、活血组和福善美组。测定小鼠骨骼、骨骼肌α-KGDHC含量变化。结果:与正常组比较,模型组、健脾组、活血组小鼠骨骼、骨骼肌的α-KGDHC显著降低(P<0.01);与模型组比较,补肾组和福善美组小鼠骨骼、骨骼肌的α-KGDHC明显升高(P<0.01)。结论:①骨质疏松症的形成,可能与骨骼、骨骼肌α-KGDHC含量异常变化有关。②补肾、健脾方法通过提高骨质疏松症小鼠的骨骼、骨骼肌α-KGDHC,对骨质疏松症具有一定的防治作用。

Objective： To observe the mechanism of different traditional Chinese medicine in treating osteoporosis by investigating expression of ct-KGDHC in the skeleton and skeletal muscle of osteoporosis mice. Methods： Besides the WT group, OPG knockout mice were taken as the osteoporosis model. OPG-/- mice were randomly divided into model control group （no herb inducted）, invigorating kidney group, invigorating spleen group, promoting blood flow group and Fosamax group. Euzymelinked immunosorbent assay were taken to analyze the content of α-KGDHC in skeletal and muscle. Results： α-KGDHC in the muscle of model group, invigorating spleen group and promoting blood group mice shown significantly lower than the WT group （P〈0.01）. However, the invigorating spleen group and Fosamax group＇s content of α-KGDHC significantly increased （P〈0.01）. Conclusion： ①The lack of α-KGDHC partially is able to get the osteoporosis.②Treatment of invigorating kidney and invigorating spleen shows effective in curing osteoporosis probably by regulating α-KGDHC in the skeleton and skeletal muscle.