铁核结构对马脾铁蛋白释放铁动力学的影响

Effect Structure of Iron Core on Kinetics of Iron Release from Horse Spleen Ferritin

建立H+参与马脾铁蛋白释放铁的动力学方程。H+以 1 / 2级反应方式参与铁蛋白释放铁核表层的铁。在酸性介质 ( pH 6 5)中 ,铁蛋白释放铁的总平均速率 ( 332Fe3+/HSF·min)比在碱性介质 ( pH8 0 )中释放铁的总平均速率 ( 73Fe3+/HSF·min)高 4 6倍。铁蛋白的铁核结构和外加的磷酸盐均能影响该蛋白释放铁的速率 ,但并不改变其反应级数。

A Kinetic equation of iron release with H + existing was established from horse spleen ferritin. H + as a form of half\|order reaction took part in the reaction that the ferritin released it on the surface of iron core. Average rate of iron release (332 Fe 3+ /HSF·min) in the acid\|medium (pH 6 5) was higher 4 6 times than that (73 Fe 3+ /HSF·min) in basic\|medium (pH 8 0). The structure of iron core from horse spleen ferritin and the extra phosphate had an effect upon the rate of iron release, but the reaction order was not changed.