银杏达莫注射液降低家兔动脉血压机制研究

Ginkgo Leaf Extract and Dipyridamole Injection Reducing Arterial Blood Pressure of Rabbits in Vivo

目的:研究银杏达莫注射液(Ginkgo leaf extract and dipyridamole injection,GLED)降低家兔动脉血压的作用及机制。方法 :采用颈总动脉插管法测动脉血压。经静脉累积注射GLED(0.35、0.7、1.4、2.8、5.6、11.2 mg/kg)观察血压的变化。并分为:左旋硝基精氨酸(10 mg/kg)、异搏定(10μg/kg)、普萘洛尔(0.5 mg/kg)、吲哚美辛(0.5 mg/kg)、缬沙坦(1.5 mg/kg)5组,观察它们对GLED(5.6 mg/kg)降压作用的影响。结果 :GLED能显著降低家兔动脉血压,并具有量效依赖性(收缩压:r=0.76,t=8.4,P<0.01;舒张压:r=0.76,t=6.5,P<0.01)。静脉注射10 mg/kg左旋硝基精氨酸后,GLED(5.6 mg/kg)的降压作用明显减弱,异搏定、普萘洛尔、吲哚美辛和缬沙坦对GLED(5.6 mg/kg)的降压作用无明显影响。结论 :GLED能显著降低家兔动脉血压,其机制可能与内皮释放的NO有关。

Objective： To study Ginkgo Leaf Extract and Dipyridamole Injection （ GLED ） reducing arterial blood pressure of rabbits in vivo and the possible mechanism. Methods： Rabbits common carotid artery was intubated and arterial blood pressure in vivo was recorded. GLED with cumulative concentrations of 0.35,0.7,1.4,2.8,5.6,11.2 mg/kg was injected into vein and the changes of arterial blood pressure were observed. In addition, rabbits were divided into five groups, including 10 mg/kg N co - nitro - L - arginine （L- NN A ）, 10 f.L g/kg verapamil （Vera）, 0.5 mg/kg propranolol （Prop）, 0.5 mg/kg indomethacin （Indo）, 1.5 mg/kg valsartanal （Vals handom. 5.6 mg/kg GLED was injected into vein respectively and the changes of arterial blood pressure were observed. Results： GLED had a significant concentration-dependent negative effect on arterial blood pressure in vivo （the systolic pressure： r=0.76, t=8.4, P〈O.OI ; the diastolic pressure： r=0.76, t=6.5, P〈O.OI ）. The effect of the decreasing arterial blood pressure of GLED （5.6 mg/ kg） was significantly reduced by injection into vein with 10 mg/kg L-NNA, but there was no obvious effect by treatments respectively with Vera, Prop, Indo or Vals. Conclusion： GLED can reduce arterial blood pressure significantly in vivo. The mechanism of reducing arterial blood pressure may be related to NO releasing from endothelial cells.