HPLC同时测定四正颗粒中厚朴酚、和厚朴酚含量

Determination of Magnolol and Honokiol in Sizheng Granules by HPLC

目的：建立HPLC同时测定四正颗粒中厚朴酚、和厚朴酚的含量测定方法。方法：采用Kromasil ODS—C18柱（4．6mm×250mm，5μm），流动相甲醇－异丙醇－1％磷酸溶液（36：21：40），流速1．0mL／min，检测波长为294nm，柱温35℃。结果：厚朴酚在0．2616-1．5696μg（r=0．9999），和厚朴酚在0．1282-0．7692μg（r=0．9999）呈良好的线性关系，平均回收率厚朴酚为99．01％，RSD为1．37％（n=6），和厚朴酚为99．02％，RSD为1．41％（n=6）。结论：本法准确可靠，重复性好，可用于四正颗粒的质量控制。

Objective： To establish an HPLC method for the determinations of magnolol and honokiol in Sizheng Granules. Method： The Kromasil CI8 column was used with a mobile phase of methanolisopropanol= l % phosphoric acid with the flow rate of 1.0 mUmin. The column temperature was 35 OC and UV detection wavelength was at 294 nm. Result： The linear ranges of magnolol and honokiol were 0.2616-1.5696 f.L g （r=0.9999） and 0.1282-0.7692 μg （r=0.9999）, respectively. The average recoveries of two components were 99.01 % with RSD 1.37% （n=：o6 ）and 99.02% with RSD 1.41 % （n=6）. Conclusion： , The method is accurate, reliable and repeatable. It can be a method for quality control of Sizheng Granules.