摘要
分离群体的构建及其合理性评估是遗传作图等基因组研究的基础。利用SSRs标记对以科丰1号为母本、南农1138-2为父本构建的大豆RILs群体NJRIKY进行了分析评估。随机选取覆盖大豆基因组的138对SSRs引物对两个亲本进行多态性筛选分析，86对具有多态性，多态率高达约62.32%，共计90个多态位点。进一步利用具多态性的SSRs位点对供试群体的分析表明不仅绝大多数位点符合11分离比，而且各家系也趋于纯合。该RILs群体各家系的基因型组成近似正态分布，是一个适于遗传作图及其他基因组研究理想的RILs群体。
The prerequisite of genomic research such as genetic mapping is construction of a suitable segregating population and its reasonable evaluation before use. A recombinant inbred line (RILs), NJRIKY, was constructed through a cross between Kefeng 1 and Nannong1138-2. The present study was focused on evaluation of genetic structure of the RILs through the simple sequence repeats (SSRs) analysis. A total number of 138 pairs of SSRs primers covering the whole soybean genome were randomly selected for polymorphic analysis between two parents. 86 pairs of which were polymorphic and polymorphic rate is up to 62.32%. 90 polymorphic loci were found. Further analysis with polymorphic SSRs loci on the sampled lines indicated that not only segregation of most SSRs loci conformed to the ratio of 11, but also almost each sampled line tended to homozyge. The distribution of genotypic composition was normal. The population is ideal for genetic mapping and other genomic research.
基金
国家重点基础研究计划项目资助!(No.G1998010208
G1998010206)&&
关键词
大豆
重组近交系
微卫星标记
群体评估
soybean (Glycine max L.)
recombinant inbred line
microsatellite marker
population evaluation
