ELISA法和MEIA法测定乙肝病毒血清标志物的比较研究

Comparative study on ELISA and MEIA methods used in detecting serum HBV markers

目的:比较ELISA法和MEIA法检测乙肝五项血清标志物的检测性能。方法:用ELISA法(酶联免疫吸附法)和EMIA法(微粒子化学发光酶免疫分析)对HBV血清标志物进行乙肝五项的检测,并对强阳性标本进行稀释检测。结果:对100例患者的血清进行乙肝五项的检测,ELISA法和MEIA法对HBsAb、HBsAb、HBeAg三项标志物的检出率无显著差异(P>0.05),但对HBeAb和HBcAb的检测,MEIA法检出率明显高于ELISA法(P<0.01和P<0.05)。结论:对于乙肝病毒血清标志物的检测,MEIA法检测血清乙肝标志物的灵敏度和自动化程度均比ELISA法高,同时可动态观察疗效和监测病情。

Objective：To compare quality of ELISA and MEIA methods used in detecting serum HBV markers. Methods：ELISA and MEIA methods were used to detect serum HBV markers including HBsAg、HBsAb、HBeAg、HBeAb、HBcAb,and test the minimum dilutions. Result：The result is that the rate of detection of HBsAg、HBsAb、HBeAg are have no distinct difference determined directly by ELISA and MEIA, but the rate of detection of HBeAb determined by MEIA is higher than that deter-mined by ELISA（P＆lt;0.001）, and the rate of detection of HBcAb is have distinct difference （P＆lt;0.05）. Conclusions：The degree of sensitive and automation determined by MEIA are higher than that of ELISA,at the same time the curative effect of patients is be easily dynamic observed and the state of illness is also be easily in-spected by the method of MEIA.