摘要
8种甜荞种子表面消毒所适用的消毒剂和处理时间有所不同。 1 0 %H2 O2 溶液处理1 50min ,对榆 3- 3、黎麻道、浦江荞、龙山荞和富源红花荞的种子有很好的消毒作用 ,并且处理以后种子仍具有很高的萌发率。 862 1、B大粒和美国荞种子的消毒以 0 .1 %的HgCl2 处理 1 2min效果为好。在无菌苗子叶和下胚轴外植体脱分化形成愈伤组织的过程中 ,2 ,4-D起主要作用 ,BA起独特的作用。BA与 2 ,4-D配合使用对于愈伤组织的诱导和增殖具有良好的促进作用。高浓度的 2 ,4-D或低浓度的 2 ,4-D与BA配合使用都可抑制愈伤组织分化根。
In this paper,it were studied that effect of H 2O 2 and HgCl 2 solution to sterilization of common buckwheat seeds and phytohormone affect to induction and propagation of callus. Contamination rate were from 0 to 6.2% and germination rate were above 92% after seeds of 5 varieties (Yu3-3,Limadao,Pujiang,Longshan and Fuyuanhonghua) were sterilized with 10% H 2O 2 solution for 150min.It was applicable to seeds of 3 varieties (8621,B-Dali and America) to sterilize with 0.1% HgCl 2 solution for 12min, contamination rate of seeds were 0 and germination rate were from 56.1% to 68.7%. On media supplemented with 0.5 ～4.0mg/L 2,4-D,cotyledon and hypocotyl explants from sterilized seedlings were dedifferentiated and calli were formed. Use of BA was very importent in medium containing 2,4-D ,it was helpful for propagation of callus and was unfavourable for differentiation of roots.
基金
山西省自然科学基金!资助项目 (96 10 2 0 )
