ABA对红锥、苦槠种子发育和萌发的效应研究

Study ABA sensitivity of seeds during development of Castanopsis hystrix and Castanopsis sclerophylla

研究采用酶联免疫分析法 ( EL ISA)提取和测定红锥和苦槠发育过程中果实内 ABA含量 ,并用不同浓度 ABA喂养 ,测定果实不同发育期对外源 ABA的敏感性。用放射性同位素法测定 ABA对贮藏蛋白质的积累作用 ,结果表明 :随着红锥和苦槠种子发育过程内源ABA含量逐渐上升 ,达到高峰的时间分别为 1 1月 5日和 1 0月 5日 ,随后 ABA含量均逐渐下降。成熟采收时果实内 ABA含量比高峰时小 8～ 9倍。随着果实的发育种子对外加 ABA的敏感性逐渐变小 ,外源 ABA浓度 1 0 - 4mol/ L可以完全抑制 1 1月 2 5日 (红锥 )和 1 0月 1 5日 (苦槠 )前种子发芽 ,但对成熟的种子 1 0 - 2 mol/ L ABA亦不能抑制发芽。ABA对成熟前期胚贮藏蛋白质合成无影响 ,但能促进成熟中后期胚的贮藏蛋白质的积累作用 ,ABA维持红锥和苦槠贮藏蛋白质合成和积累作用表现在转录水平上。

This contribution dealt with the determination of ABA contents during different seed developmental stages of C.hystrix and C.sclerophylla by the method of ELISA,sensitivity of seed to different concentration of the breeded exogenous ABA,and accumulative reaction to storage protein by the means of radial isotopic tracing.Results showed that,with the development of C.hystrix and C.sclerophylla seeds,endogenous ABA contents increased obviously,then both peaked by 1 659 ng/g ( C.hystrix ) and \{1 471 ng/g\} ( C.sclerophylla ) on 5,November or October,and at last decreased until it just was one eighth or ninth of the ABA peaks when the seeds fully matured.As the sensitivity to exogenous ABA decreased during development,10 -4 mol·L -1 ABA could inhibit the germination seeds in which ABA contents could not reach peak,however,even 10 -2 mol·L -1 ABA could not inhibit the germination of the fully matured seeds.ABA content had no effects on synthesis and accumulation of storage protein in the early developmental stage,but accelerated them in the middle and late.ABA in regulation the synthesis and accumulation of storage proteins was reflected by the transcriptional level.