三七总皂苷对脑出血大鼠脑含水量和凝血酶的影响

Effect of Panax Notoginseng Saponins on Cerebral Water Content and Thrombin of Intracerebral Hemorrhage Rats

目的观察三七总皂苷对脑出血大鼠脑组织含水量及凝血酶的影响,探讨其对脑出血的作用机制。方法 SD雄性大鼠随机分为空白组、假手术组、模型组、治疗组,分别观察6、24、48、72 h及7 d 5个时间点。采用尾壳核注入胶原酶肝素钠生理盐水溶液法建立大鼠脑出血模型,治疗组给予三七总皂苷腹腔注射,每日1次,采用干湿重法测量脑组织含水量,检测48 h纤维蛋白原含量(FIB)和凝血酶凝结时间(TT)。结果各时点模型组脑含水量与空白组、假手术组比较明显升高,差异有统计学意义(P<0.05);除6 h时点外,治疗组各时点与模型组和治疗组比较,脑含水量明显降低,差异有统计学意义(P<0.05)。与模型组比较,治疗组FIB明显降低,差异有统计学意义(P<0.05);治疗组TT下降不明显,差异无统计学意义(P>0.05)。结论三七总皂苷通过下调脑出血大鼠脑组织含水量、FIB含量及TT达到治疗脑出血的目的。

Objective To investigate the effects of Panax notoginseng saponins （PNS） on the cerebral water content and thrombin in the rats with intracerebral hemorrhage （ICH）, and explore its mechnism for treating ICH. Methods SD male rats were randomly divided into blank group, sham operation group, model group and treatment group, and time points of 6, 24, 48, 72 h and 7 d were observed. ICH rat model was induced by collagenase and heparin in saline solution injected into caudate nucleus. The treatment group was injected PNS intraperitoneally once a day, and the cerebral water content was assessed by dry-wet weight method. Both content of fibrinogen （FIB） and thrombin clotting time （TT） in 48 h were measured. Results The cerebral water content of model group increased at every time point compared with blank group and sham operation group （P〈 0.05）. The cerebral water content of treatment group was significantly decreased at every time point except for 6 h compared with model group （P〈0.05）. FIB of treatment group decreased compared with model group （P 〈 0.05）, while TT of treatment group was not significantly decreased （P〉0.05）. Conclusion PNS may play a role in treating ICH by decreasing cerebral water content, FIB and TT of ICH rats.