一种产血纤维蛋白溶酶菌株的筛选及液体发酵条件的选择

SELECTION OF FERMENT MEDIUM FOR THE SECRETION OF PLASMIN FROM A MARINE BACTERIA

主要进行了产生纤维蛋白溶酶菌株的筛选培养和液体发酵条件的优化等方面的研究工作。结果表明 ,该菌为一种短杆状菌 ,革兰氏染色为阴性 ,具有对血纤维蛋白的产酶依赖性。用此菌作为菌种进行发酵产酶的最适条件为 :碳源 ,木糖1.5% ;氮源 ,血纤维蛋白1.75% ;发酵液初始 pH7.4 ;接种量5% ;发酵时间24h。

This paper reports for the optimum growth medium for the secretion of plasmin from a marine bacteria named FE 92 8. This bacteria is shortly bacillary, gram negative, dependant on the existence of fibrin. The quantity of this bacteria and the activity of plasmin secreted by the bacteria is the highest at below culture condition: carbon source, 1.5 percent xylose; nutrogen source, 1.75 percent fibrin; initial pH 7.4;inoculative quantity,5.0 percent, ferment temperature, 30 centigrade degree, ferment time,24hours.