牙鲆迟缓爱德华氏菌急性感染实验:4种不同方法的比较

INFECTION EXPERIMENTS WITH Edwardsiella tarda IN THE BASTARD HALIBUT (Paralichthys olivaceus): AN EVALUATION OF FOUR DIFFERENT CHALLENGE METHODS

为了检验疫苗对牙鲆免疫效果 ,探讨有效的注射途径尤为重要。本文通过采用迟缓爱德华氏菌 (Edwardsiellatarda)对牙鲆 (Paralichthysolivaceus)进行感染实验 ,对口腔、肌肉、腹腔和腹腔加肌肉4种注射方法进行了评价比较。其半致死浓度 (LD50)分别为口腔灌注法108.38CFU/ml、肌肉注射法107.54CFU/ml、腹腔注射法106.70CFU/ml和腹腔加肌肉注射法106.09CFU/ml。结果表明牙鲆对迟缓爱德华氏菌敏感 ,在这4种注射方法中 。

In testing the efficacy of vaccines in the Bastard halibut(Paralichthys olivaceas),a reliable challenge method is essential.Four different challenge methods with Edwardsiella tarda,including oral administration, intramuscular injection, intraperitoneal injection,and both intramuscular and intraperitoneal injection methods were evaluated in the Bastard halibut.LD 50 were determined as 10 7.54 CUF/ml by intramuscular injection, 10 6.70 CUF/ml by intraperitoneal injection, 10 8.38 CUF/ml by oral administration, 10 6.09 CUF/ml by intramuscular and intraperitoneal injection. These results show that the Bastard halibut has high susceptibility to Edwardsiell tarda. Campare to the other three methods, intraperitoneal injection method is the most suitable method with high mortalities.