Cu^(2+)、Zn^(2+)诱导稀有鮈鲫应激蛋白质的研究

THE INDUCTION OF STRESS PROTEIN IN GROBIOCYPRIS RARUS BY Cu^(2+)AND Zn^(2+)

以稀有鲫为材料 ,研究了应激蛋白质作为生物学指标的敏感性。结果表明 ,在无可观察效应浓度下 ,经 5d亚慢性胁迫暴露 ,以Cu2 +为胁迫因子 ,稀有鲫被诱导出约 54KDa的应激蛋白质 ;以Zn2 +为胁迫因子 ,稀有鲫被诱导出约 94KDa ,67KDa和 40KDa的应激蛋白质。应激蛋白质有可能成为一种生物学指标运用于生态风险性早期预警。

The sensitivity of stress protein as a biomarker was researched in the project. The experiment was initiated using ＜3d old Grobiocypris rarus larvae according to subchronic toxicity test and run for 5d. Special stress proteins of approximately 54KDa or 94KDa, 67KDa and 40KDa were induced in response to Cu2+ or Zn2+ at the No Observable Effect Concentration, respectively. Compared with the conventional end points such as growth, this assay is a more sensitive and rapid indicator. Therefore, stress protein might be an useful biomarker as an early warning system in risk assessment.