摘要
从耐热性极强的酿酒酵母菌株AS2 1 41 6中分离纯化出总RNA和mRNA ,以AMV逆转录酶合成cDNA ,采用保守引物 ,从该cDNA中扩增克隆出tps1基因 ,对该基因的全序列分析表明 ,该基因含有 1 50 7个核苷酸 ,与国外报道相关基因的同源性达 99 6%。利用BamHⅠ和SacⅠ切点将tps1基因插入植物表达载体 pBin438多克隆位点上 ,得到tps1基因植物表达载体重组质粒。
Total RNA,mRNA were isolated from baker's yeast S.cerevisiae , the cDNA was prepared with AMV Reverse Transcriptase from the total mRNA.The gene of trehalose \|6\|phosphate synthase was cloned form the cDNA with PCR amplification. The gene was sequenced and the results showed that the tpsl gene contains 1507 nucleotides and 99.6% identity with S.cerevisiae . The tps1 gene was constructed on the plant expression vector pBin438.
出处
《微生物学报》
CAS
CSCD
北大核心
2001年第1期54-58,共5页
Acta Microbiologica Sinica
基金
国家自然基金资助项目!( 39570 4 0 3)&&
