深层海水对小鼠创面愈合的促进作用

The influence of deep sea water on wound healing of mice

目的探讨"深层海水"对创面修复的作用,为创面修复提供新的方法。方法选取4周龄昆明种雄性小鼠24只,分为实验组(深层海水)12只和对照组(自来水)12只,分别喂养14d并计算饮水、饮食量。第15天在小鼠背部建立1cm×1cm面积创面,术后仍以原方案喂养,检测创口愈合速度。术后第3、5、7天取创缘组织,行创面病理学观察。结果两组小鼠饮食、饮水总量无明显差别,自术后第5天,深层海水组创面愈合率明显高于自来水组,差异有统计学意义(P<0.05)。组织病理学观察,深层海水组与自来水组比较,新生的肉芽组织中血管内皮细胞和新生毛细血管增多,炎性细胞浸润较少,成纤维细胞增生明显。结论深层海水可以促进小鼠创面的愈合。

Objective This experiment aimed to study the influence of deep sea water （DSW） on wound healing of mice .Methods 24 Mice were randomly divided into two groups ：group DSW（n=12） and group sterilize tap water（STW）（n=12） ,freely feeding for 14 days respectively ,and calculated the amount of food and water .On the 15th day ,1 cm ×1 cm size of wound was established on the back area of mice ,and continued to feed with DSW and STW respectively .Tracking the wound healing rate .Specimen was taken in the edge of wound tissue on postoperative 3 ,5 ,7 days ,then observed histopathological changes .Results Compared group DSW with group STW ,there was no significant difference in the total amount of food and water .5 days after the formation of wounds ,the wound healing rate of group DSW was significantly higher than group STW .Histological observation ：compared with group STW ,vascular endothelial cells and new capillaries of the group DSW was increased ,and group DSW had less inflammatory cell and more fibroblast cells proliferation .Conclusion deep sea water can promote wound healing .