摘要
用中国春ｐｈｌｂ突变体（Ｃ．Ｓ ｐｈｉｂｐｈｌｂ）与普通小麦一簇毛麦６Ｖ（６Ａ）异代换系（Ｓｕｂ．６Ｖ）杂交，再用ｐｈｉｂ突变体与Ｆ１回交，在高配对植株中筛选出一个６Ｖ染色体发生变化的植株，编号为ＬＶ０２。用染色体Ｃ－分带和荧光原位杂交技术，对ＬＶ０２株系的后代进一步鉴定，在ＢＣ１Ｆ２中筛选鉴定出１株编号为ＬＶ０２－０１的植株，该株含有４０条普通小麦染色体，１条簇毛麦６Ｖ染色体和１条６Ｖ短臂端着丝粒染色体。在ＬＶ０２－０１的分离后代中用同样技术鉴定出８株普通小麦－簇毛麦６ＶＳ端二体代换系。
Chinese Spring phlb mutant (C S phlbphib) was crossed to Triticum aestivum-Haynaldia villosa 6V (6A) alien substitution line and F1 bask was crossed with C.S phlbphlb. One LV02 with varied H villosa 6V chromosome and one LV 02-01 with 40 T aestivum chromosome, one H villosa 6V and 6VS chromosome were screened in BC1F1 and BC1F2 respectively by C-banding and the fluorescence in situ hybridization (HSH). In segregated generation of LV02-01, eight T aestivum-H. villosa 6VS ditelosomic substitution lines were screened by ASH and C-banding.
基金
863计划资助
国家自然科学基金
