摘要
为研究BHK-21细胞的生长及代谢动力学特征,试验采用低密度静置培养方法,每天测定细胞生长密度和活力,并利用多参数生化分析仪测定培养液中Gluc、Lac、Gln和NH4+的含量,计算细胞指数生长期的比代谢速率。结果表明:BHK-21细胞生长曲线呈S型,最大增殖密度为50.3×104 upf·mL-1,倍增时间为21.1h;对数生长期Gluc和Gln的比消耗速率为-3.79mg·(106 cells)-1·d-1和-9.95μmol·(106 cells)-1·d-1,Lac和NH4+的比生成速率为2.81mg·(106 cells)-1·d-1和6.30μmol·(106 cells)-1·d-1。
In order to research the grow th and metabolism kinetic characteristics of BHK-21 cells ,low cell densi-ty cultured method was used to determine cell density and vitality every day .The contents of Gluc ,Lac ,Gln , and NH4 ＋ in medium were determined by multi-parameter biochemical analyzer ,and the specific metabolic rate were calculated .The results showed that the growth curve of BHK-21 cells was “S” type ,the largest density was 50 .3 × 104 upf·mL-1 and the doubling time was 21 .1 h .Specific consumption rate of Gluc and Gln were-3 .79 mg·（106 cells）-1 ·d-1 and -9 .95μmol·（106 cells）-1 ·d-1 in logarithmic phase ,and specific generation rate of Lac and NH4 ＋ were 2 .81 mg·（106 cells）-1·d-1 and 6 .30 μmol·（106 cells）-1·d-1 .
出处
《黑龙江农业科学》
2014年第2期62-64,共3页
Heilongjiang Agricultural Sciences
基金
甘肃省科技重大专项资助项目(1203FKDA030)
西北民族大学中央高校基金资助项目(31920130066)
关键词
BHK-21细胞
生长特征
代谢动力学
比代谢速率
比消耗速率
比生成速率
BHK-21 cell
growth characteristics
metabolism kinetics
specific metabolic rate
specific consump-tion rate
specific generation rate
