摘要
We studied some factors affecting the lipase production from candida rugosa, they mainly included medium compositions and culture condition.The result showed that the optimal medium compositions for lipase production are 0.1% glucose 4.0% olive oil (carbon source),0.3% NH 4NO 3(nitrogen source),1.2%K 2HPO 4 and 0.4%MgSO 4·7H 2O.And the optimal culture condition is initial pH6.5,temperature 30℃,agitation 180r/min and time 60h. As a result, and the lipase activity could reach 19.5u/mL.Meanwhile we found that the surfactant could be helpful to the lipase production, and the optimal surfactant concentration was 0.03% GPE.The lipase activity was improved by more than 170% after we optimized the medium compositions and culture condition.While in a 5L fermentator, the lipase activity of fermentation broth could reach 33.5u/mL within 48 hours.
We studied some factors affecting the lipase production from candida rugosa, they mainly included medium compositions and culture condition.The result showed that the optimal medium compositions for lipase production are 0.1% glucose 4.0% olive oil (carbon source),0.3% NH 4NO 3(nitrogen source),1.2%K 2HPO 4 and 0.4%MgSO 4·7H 2O.And the optimal culture condition is initial pH6.5,temperature 30℃,agitation 180r/min and time 60h. As a result, and the lipase activity could reach 19.5u/mL.Meanwhile we found that the surfactant could be helpful to the lipase production, and the optimal surfactant concentration was 0.03% GPE.The lipase activity was improved by more than 170% after we optimized the medium compositions and culture condition.While in a 5L fermentator, the lipase activity of fermentation broth could reach 33.5u/mL within 48 hours.
出处
《生物工程学报》
CAS
CSCD
北大核心
2001年第1期101-104,共4页
Chinese Journal of Biotechnology
基金
国家"九五"攻关项目!(96 C0 3 0 2 0 5 )
上海市科委攻关项目!(98431912 2 )
国家自然科学基金!(2 970 6 0 0 3)资助项目&&
