摘要
目的 :比较不同地株微小按蚊核糖体DNA(rDNA)内转录间隔 2区 (ITS2 )序列差异。方法 :通过对PCR扩增rDNAITS2片段测序 ,比较其不同地株差异。结果 :对 6株微小按蚊测序比较 ,存在有微小按蚊A和C型。结论 :rDNAITS2序列差异可用于建立A。
Objective:To compare the sequence difference of the ribosomal DNA second internal transcribed spacer (ITS2) in the different strains of Anopheles minimus in Yunnan. Methods: The PCR amplified rDNA ITS2 sequences, to compare the variation of An. minimus . Results: The different strains of 6 populations were sequenced, which could be separate into Anopheles minimus A and C. Conclusion: The sequence difference of the rDNA ITS2 would be very useful for the developing a molecular approach to distinguish An. minimus A and C.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2001年第1期24-27,共4页
Chinese Journal of Vector Biology and Control
关键词
微波按蚊
DNA
核糖体
第2内转录间隔区
Anopheles minimus
DNA
Ribosomal
Second internal transcribed spacer
