合肥市HIV/AIDS未治疗人群的耐药基因及亚型分析

Study of HIV-1 subtype diversity and drug resistance among antiretroviral treatment-naive patients in Hefei district

目的了解合肥地区HIV/AIDS未治疗人群基因亚型和原发性耐药情况。方法应用巢式RT-PCR扩增HIV-1 pol区聚合酶基因,经DNA测序后进行系统进化树分析和耐药性分析。结果收集79例合肥地区HIV/AIDS未治疗者,经RT-PCR扩增并获得有效基因亚型序列65例,基因分型以CRF01-AE重组亚型最多39例(60.0%);其次依次是CRF07-BC 21例(32.3%)、B亚型4例(6.2%)和CRF08-BC1例(1.5%)。1例蛋白酶抑制剂(PIs)主要耐药基因突变,2例反转录酶抑制剂(RTIs)耐药基因突变。总耐药突变率为4.6%(3/65)。结论当前合肥市主要的HIV-1流行亚型是CRF01-AE和CRF07-BC,HIV原发性耐药的发生率在国内处于较低水平。

Objective To understand the genetic subtypes and drug resistance associated mutations of HIV-1 stains currently circulating among antiretroviral treatment-naive patients in Hefei.Methods Seventy-nine HIV-1-infected treatment-naive individuals living in Hefei were sampled and their plasma was collected.HIV-1 pol gene were amplified by nest RT-PCR,sequenced and phylogenetically analyzed.Drug-resistance associated mutations in protease and reverse transcriptase regions were analyzed with Standford University HIV/AIDS Drug Resistance Database.Results CRF01-AE predominated in Hefei with 60.0%,followed by CRF07-BC（32.3%）,subtype B（6.2%）,CRF08-BC（1.5%）.The mutation conferring primary and secondary resistance to protease inhibitors（PIs） were detected in 1（1.5%） of 65 treatment-naive individuals; whereas the mutations conferring resistance to reverse transcriptase inhibitors（RTIs） were identified in 2（3.1%） of 65 treatment-naive individuals.The whole rate of drug resistance mutation was about 4.6%（3/65）.Conclusions Major genetic subtypes of HIV-1 in Hefei at present were CRF01-AE and CRF07-BC.The prevalence of genotypic drug resistance was low among treatment-naive HIV-1 infected patient in Hefei.