花分生组织决定基因AP1转化矮牵牛的研究

Studies on Petunia hybrida Transformed with Flower_meristem_identity Gene AP1

利用RT_PCR方法从拟南芥 (Arabidopsisthaliana (L .)Heynh .)中克隆了花分生组织决定基因 (flower_meristem_identitygene)AP1,进行了全序列测定。测序结果显示 ,所得到的基因与发表的序列仅有一个碱基的差异 ,但并不影响蛋白质的一级结构。将AP1基因克隆入植物中间载体p2 0 8,通过根癌土壤杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化矮牵牛 (PetuniahybridaVilm .)。对转基因植株进行了PCR和Southern检测 ,所得到的两个株系的转基因矮牵牛在R0 代即表现出提前且持续不断地开花的特性 。

Flower_meristem_identity gene AP1 was isolated from Arabidopsis thaliana (L.) Heynh. by RT_PCR. The result of the sequencing showed that only a single nucleotide change was found in this AP1 gene compared with the published sequence, but this changed nucleotide did not effect the primary structure of the protein. Driven by the CaMV 35S promoter, AP1 was stably integrated into the genome of Petunia hybrida Vilm. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated transformation. The intergration of the transgene was identified by PCR and Southern blot analysis. The R 0 generation of two transgenic lines could flower earlier and constantly even in the tissue culture flask, differing from the non_transformed Petunia hybrida .