响应面法优化金黄色葡萄球菌生物被膜的培养条件

Optimization of Culture Conditions for Staphylococcus Aureus Biofilm by Response Surface Methodology

以食源性病原菌金黄色葡萄球菌形成的生物被膜为研究对象,采用响应曲面分析法(RSA)对其培养条件进行优化。结果表明,金黄色葡萄球菌生物被膜培养的最佳条件:培养基pH 7,菌悬液的初始悬浮菌浓度为108CFU/mL,培养温度37℃,培养24 h。此条件下金黄色葡萄球菌生物被膜的活菌量可达5.60 Lgcfu/cm2。

This paper was mainly studied on biofilm formed by the foodborne pathogenic bacteria Staphylococcus au- reus, the optimum technology conditions of cultivate Staphylococcus aureus biofilm was optimized by response surface methodology. The results showed that the optimum culture condition were as follows： culture medium pH was 7, initial concentration of bacteriologic was 10^8 CFU/mL, cultivating temperature was 37℃ for 24h, Staphylococcus aureus biofilm colony count could reach 5.66Lgcfu/cm2.