大米、糙米蛋白及其酶解产物的体外降脂研究

Study on in Vitro Hypocholesterolemic Effects of White Rice Protein,Brown Rice Protein,Soybean Protein and Their Hydrolysates

研究不同模拟体系中大米、糙米和大豆蛋白及其Alcalase 2.4L酶解产物降低胆固醇和束缚胆汁酸的生物活性。在胆固醇胶束溶液中,大米蛋白（WRP）的降胆固醇能力最低（9.7%）,而大豆蛋白酶解产物（SPH）具有最高的降低胆固醇活性（18%）。在模拟胆汁酸体系中,蛋白质及其酶解产物对胆汁酸的束缚率为10%~16%,它们可在不同程度上抑制Caco-2细胞对胶束溶液中胆固醇的吸收（11%~20%）。

The hypocholesterolemic effects of white rice protein（WRP）,brown rice protein（BRP）,soybean protein（SP）,and their hydrolysates（WRPH,BRPH,and SPH） were identified using cholesterol micellar solution and Caco-2 cell.The bile acid binding ability was indentified using artificial bile acid solution.In cholesterol micellar solution,WRP had the lowest cholesterol-lowering ability（9.7%） and SPH had the highest（18%）;all the proteins and their hydrolysates had the different bile-acid binding abilities（10% -16%）;in Caco-2 cell,the cholesterol-lowering abilities of WRP,BRP,SP and their hydrolysates were 11%-20%.