凯力康对大鼠心肌缺血再灌注损伤的保护作用

The Protective Function of Kailikang in Ischemia-reperfusion(I/R)Injury of Myocardium in Rats

目的:探讨凯力康对大鼠心肌缺血再灌注(I/R)损伤的保护作用及作用机理。方法:SD大鼠50只随机分为假手术组、模型组、凯力康15×10^(-3)PAN U/Kg组、凯力康30x10^(-3)PAN U/Kg组及硝酸山梨酯组(1.0 mg/kg),每组10只。大鼠麻醉后测量左心室内压,记录体表心电图;随后开胸结扎左前降支冠状动脉,造成缺血后30 min再灌注120 min(假手术组除外)。结扎后各组舌下静脉给药,记录缺血前、缺血后及灌注后各项血流动力学指标;测定血清中乳酸脱氢酶(LDH)、磷酸肌酸激酶(CK-MB)、超氧化物歧化酶(SOD)、丙二醛(MDA);计算心脏指数及心肌梗死范围。结果:与模型组相比,凯力康对I,/R大鼠的血流动力学指标均有明显的改善作用;能缩小心肌梗死范围;降低血清中LDH、CK、MDA水平、升高血清中SOD活性。结论:凯力康通过提高血清中SOD含量、降低MAD、LDH、CK含量而对大鼠心肌I/R损伤有明显的保护作用。

Objective：To investigate the protective function of kailikang in ischemia-reperfusion （I/R） injury of myocardium in rats and its mechanisms. Methods： Fifty Sprague-Dawley rats were assigned randomly to five groups, and ten rats were incorporated in each group as below：group I：sham control （without LAD ligation）, group I1： I/R injury without treatment,group III： I/R injury with kailikang 15×10-3 PANU/Kg,group IV：I/R injury with kailikang 15×10-3 PANU/Kg, group V：I/R injury with isosorbide dinitrate 1.0 mg/kg. Left ventricular pressure of the rats were measured post anesthetization, and the surface electrocardiograms were recorded at the same time. The rats were then underwent ligation of the left descending coronary artery resulting in ischemia for 30 minutes and occlusion- reperfusion for 120 minutes excluding the sham control group. Drugs were administered through sublingual veins for each group followed by ligation. Hemodynamic indicators were documented before operation, after ligation and post repeffusion respectively. The level of lactic dehydrogenase （LDH）, creatine phosphate kinase （CK-MB）, superoxide dismutase （SOD）, and malonaldehyde （MAD） in the serum were detected, cardiac index and infarct size were calculated. Results： Distinct improvements in hemodynamic indicators were observed in rats treated with Kailikang compared with those of the I/R injury without treatment. There were a reduction in infarct size and a decrease of the level of LDH, CKMB and MDA in Kailikang treatment groups. In addition, the activity of SOD was boosted in the serum. Conclusion： Kailikang exerted the protective function post I/R injury of myocardium in rats by enhancing the level of SOD and diminishing the level of MAD, LDH and CKMB in the serum.