白念珠菌CaPSY2基因的敲除和功能研究

Disruption and functional characterization of CaPSY2 gene in Candida albicans

目的:研究人体病原真菌白念珠菌CaPsy2蛋白的功能,探讨它在DNA损伤检查点修复过程中所起的作用。方法:采用同源重组的方法构建CaPSY2双等位基因缺失株,并利用倍比稀释法探讨CaPSY2缺失株在含有遗传毒性试剂平板上的表型。结果:PCR检测结果显示CaPSY2双等位基因缺失株构建成功,而表型结果显示CaPsy2缺失会导致缺失株对遗传毒性试剂敏感,且这种敏感性表型可以被外源的CaPSY2基因互补。结论:CaPsy2蛋白参与DNA损伤修复过程,并且可能作为CaPph3的调节亚基而发挥作用。

Objective：To study the gene function of CaPSY2 in DNA damage checkpoint in Candida albicans. Methods：Ho-mologous recombination was used to construct the null mutant of CaPSY2 and phenotype screening was used to explore the function of CaPsy2. Results： Homozygous null mutant for CaPSY2 was constructed successfully and showed hypersensitive to genotoxicity which can be rescued by exogenous CaPsy2. Conclusion： CaPsy2 plays a role in DNA damage checkpoint path-way in Candida albicans.