急性胎兔宫内窘迫模型脑NO、iNOS和T-SOD变化实验研究

Experimental research on the changes of NO iNOS and T-SOD of brain tissue on acute intrauterine hypoxia in pregnant rabbit model

目的建立孕兔宫内窘迫模型,探讨胎兔脑一氧化氮(NO)、诱生型一氧化氮合酶(iNOS)和总超氧化物歧化酶(T-SOD)含量变化规律。方法新西兰大白孕兔30只,随机分为空白对照组(n=12)、假手术组(n=9)和宫内缺血缺氧组(n=9),宫内缺血缺氧组建立孕兔开腹子宫动脉结扎胎兔宫内窘迫缺血缺氧模型,分别开腹取各组胎兔脑匀浆液测定NO、iNOS和T-SOD含量,进行统计学比较。结果与空白对照组比较,假手术组和宫内缺血缺氧组NO、iNOS的含量显著升高(P<0.01),T-SOD的含量明显降低(P<0.01),差异具有统计学意义。与假手术组相比,宫内缺血缺氧组NO、iNOS显著升高(P<0.01),差异具有统计学意义;T-SOD降低不显著(P=0.399)。结论通过检测孕兔宫内窘迫模型NO、iNOS和T-SOD显示,胎兔缺氧应激反应强烈,手术操作及子宫动脉结扎对脑组织内自由基含量影响显著。

Objective To Study the changes of NO, iNOS and T-SOD in pregnant rabbits brain tissue after build acute intrauterine hypoxia model. Methods 30 New Zealand white pregnant rabbits were randomly divided into 3 groups, blank group（n=12）, sham operated group（n=9） and intrauterine ischemia hypoxia group（n=9）, according to uterine artery ligation and erecting uterine ischemia hypoxia models. The contents of NO, iNOS and T-SOD in pregnant rabbits brain homogenate were measured and compared. Results Compared with the blank group, the contents of NO and iNOS were significantly increased in sham operated group and intrauterine ischemia hypoxia group（P〈0.01）, the contents of T-SOD were significantly decreased（P〈0.01）. Compared with sham operated group, the contents of NO and iNOS were significantly increased in intrauterine ischemia hypoxia group（P〈0.01）, but the contents of T-SOD were not significantly decreased（P=0.399）. Conclusion All pregnant rabbits have an strong stress response, after the operative procedure and uterine artery ligation, they have an significant effect upon the contents of NO, iNOS and T-SOD in pregnant rabbits brain tissue.