双峰驼卵泡发育过程中卵泡液差异蛋白质组学

Comparative proteomics studies of Bactrian camel follicular fluid between the different periods of follicular development

为了比较双峰驼卵泡发育过程中卵泡液中差异表达的蛋白质,将卵泡按照直径分为6类,运用双向电泳技术构建双峰驼卵泡液蛋白质二维凝胶电泳图谱,凝胶经考马斯亮蓝染色后用PDQuest 8.0检测差异蛋白,结果表明在6类大小不同的卵泡中共检测到13个差异蛋白点,这些蛋白点经LC-MS/MS鉴定出7种不同的蛋白质,它们分别是:血红蛋白、toll-like受体9、抗凝血酶、聚泛素、γ纤维蛋白原、重组活化蛋白1和跨膜与卷曲螺旋域3。基于这些蛋白的功能和表达模式,结合实验结果讨论了这些蛋白质在生殖中的功能,发现toll-like受体9、聚泛素、γ纤维蛋白原和重组活化蛋白1可能与卵泡发育或卵母细胞的成熟有关。这些蛋白质的发现为了解双峰驼卵泡发育和卵母细胞成熟的生理机制奠定基础。

The aim was to identify differentially expressed proteins in the follicular fluid. Follicles were collected from the Bactrian camel （Camelus bactrianus ） and divided into six groups according to their diameter, and crude follicular fluid samples were analyzed by 2 - dimensional gel eleetrophoresis （2 - DE ）. Differential protein spots were detected by PDQuest 8.0 software after staining with commassie blue. Thirteen differentially expressed proteins were found from the six classes of developmental follicles. These spots were identified by LC-MS/MS. Seven unique proteins was found, including hemoglobin, toll-like receptor 9, antithrombin, recombination activating protein 1, polyubiquitin, transmembrane and coiled coil domain protein 3, and gamma fibrinogen. Based on existing knowledge of their functions and patterns of expres- sion, we hypothesize that toll-like receptor 9, polyubiquitin, gamma fibrinogen and recombination activating protein 1 might play significant roles in the development and maturation of oocytes, and ovarian follicular growth and development. This study provides a solid basis for further research on the processes and mechanisms which regulate follicular development and oocyte maturation in Bactrian camel.