非结核分枝杆菌肺病血清中细胞因子的检测及临床意义

Measurement and clinical significance of serum cytokines in patients with nontuberculous mycobacteria disease

目的研究血清白介素1(IL-1)、白介素2受体(IL-2R)、白介素6(IL-6)、白介素8(IL-8)、白介素10(IL-10)、肿瘤坏死因子α(TNF-α)和降钙素原(PCT)的检测在非结核分枝杆菌肺病患者的临床意义。方法采用酶联免疫方法(ELISA)检测11例非结核分枝杆菌肺病患者和14例对照组血清中IL-1、IL-2R、IL-6、IL-8、IL-10、TNF-α和PCT的水平变化。结果与对照组相比较,非结核分枝杆菌肺病组血清中TNF-α的含量明显升高(P<0.05),而IL-1 IL-2R、IL-6、IL-8、IL-10和PCT等五个细胞因子的含量无明显变化(P>0.05)。进一步分析结果显示,非结核分枝杆菌肺病组血清中TNF-α等六个细胞因子的含量变化与有否空洞无关(P>0.05)。结论血清TNF-α在非结核分枝杆菌肺病患者血清中明显升高,可作为非结核分枝杆菌肺病感染诊断的辅助指标。

Objective To explore the clinical significance of serm IL-1 , IL-2R, IL-6, IL-8, IL-10, TNF-a and PCT levels in patients with nontuberculous mycobacteria disease. Methods The serum contents of IL-1, IL-2R, IL-6, IL-8, IL-10, TNF-a and PCT were examined in 11 patients with nontuberculous mycobacteria disease and 14 healthy people as controls. Results The serum level of TNF-ct in patients with nontuberculous mycobacteria disease significantly increased compared with the controls （ P 〈 0. 05 ）. However, the serm levels of IL-I, IL-2R, IL-6, IL-8, IL-10 and PCT showed no siginificant difference （P 〉 0. 05 ）. Further analysis showed that changes of serum IL-1, IL- 2R,II,-6,IL-8,IL-10,TNF-a and PCT in patients with nontuberculous mycobacteria disease were not correlated with cavity （ P 〉 0. 05 ）. Conclusions The serum level of TNF-ain patients with nontuberculous mycobaeteria disease in- creases significantly, which can be an assistant index for nontuberculous mycobacteria disease infection diagnosis.