延边地区感染幽门螺杆菌CagA蛋白与胃癌的相关疾病的分析

目的探讨吉林省延边地区朝鲜族与汉族之间幽门螺杆菌(Helicobacter pylori Hp)细胞毒素相关基因与胃癌及癌前疾病的相关性。方法 Hp检查阳性患者571例(朝257例,汉314例),慢性浅表性胃炎组(CSG)168例(朝75例,汉93例)、慢性萎缩性胃炎组(CAG)167例(朝76例,汉91例)、胃溃疡(GU)组132例(朝60例,汉72例),胃癌组(GC)104例(朝46例,汉58例)。采用快速尿素酶法、14 C呼气试验法、免疫印迹法检测定细胞毒素相关基因蛋白(CagA)菌株感染率。结果 (1)朝鲜族与汉族CagA基因菌株阳性率比较差异有统计学意义(P=0.004<0.05)。(2)在朝鲜族组中胃癌组、胃溃疡、萎缩性胃炎组与均较浅表性胃炎组CagA基因菌株阳性率高(P<0.05)。同样,在汉族组中胃癌组、胃溃疡、萎缩性胃炎组与也均较浅表性胃炎组CagA基因菌株阳性率高(P<0.05)。(3)朝鲜族胃癌组CagA基因菌株阳性率低于汉族胃癌组(P=0.019<0.05)。结论 (1)朝鲜族CagA基因菌株阳性率较汉族患者低。(2)感染CagA基因菌株易引起严重的炎症反应,与胃癌、胃溃疡及慢性萎缩性胃炎关系密切。(3)朝鲜族患者胃癌的发生与CagA基因菌株感染相关性较汉族患者小。

Objective To study on association of Helicobacter pylori CagA gene of strain with gastric cancer and gastric precancerous disease in chao and han nationalities. Methods In 571 case （chao 257 , han 314） infected by Helicobacer pylori ,contain 172 cases chronic superficial gastritis （CSG）（chao 79, han 93） , 180 cases chronic atrophic gastritis（CAG）（chao 79, han 91）,135 cases gastric ulcer（GU）（chao 63, han 72）, 94 cases gastric cancer（GC）（chao 36, han 58）. CagA in serum were measured by innunoblotting. 14 C urea breath test, Rapid uricase tests used to identify Hp infecrion.Results （1）Positive rates of Hp CagA gene strain in han nationalities were higher than that in chao nationalities,there were difference by statistical comparison （P=0.004＆lt;0.05）.（2）Positive rates of Hp CagA gene strain in GC,CAG and GU were higher than that in CSG, there were difference by statistical comparison in chao nationalities （P＆lt;0.05）. Positive rates of Hp CagA gene strain in GC,CAG and GU were higher than that in CSG, there were difference by statistical comparison in han nationalities （P＆lt;0.05）.（3）Positive rates of Hp CagA gene strain in han nationalities were higher than that in chao nationalities with gastric cancer group,there were difference by statistical comparison （P=0.019＆lt;0.05）. Conculusion （1）Positive rates of Hp CagA gene strain in han nationalities were higher than that in chao nationalities.（2）The more serious inflammatory reaction were caused by Hp CagA gene strain with CAG, GU and GC.（3）The Hp CagA gene strain played more important roles in chao patients than in han patients of causing gastric cancer.