UPLC法同时测定吸烟者尿液中苯乙醛酸和苯乙醇酸含量

Simultaneous Determination of Phenylglyoxylic Acid and Mandelic Acid in Smoker's Urine by UPLC Method

建立了有机混合溶剂萃取、超高效液相色谱法（UPLC）同时测定尿液样本中苯乙醛酸（PGA）和苯乙醇酸（MA）含量的检测方法，并测定了50个吸烟者与非吸烟者的尿液样本。结果表明：①尿液样本经萃取后，流动相稀释直接进样，分析时间仅需8min。②PGA和MA的工作曲线浓度范围分别为0．425-21．500和1～100μg／mL时，相关系数均大于0．9995。③PGA和MA的定量限分别为0．013和0．077μg／mL，回收率分别为86．67％～96．33％和88．50％～97．17％，日内精密度均小于8％，日间精密度均小于10％。④吸烟者尿液样本中PGA和MA的含量皆高于非吸烟者，统计学分析结果表明二者之间呈现显著性差异（P〈0．05）。该方法可以简单、快速、准确地检测尿液中苯乙烯代谢物的含量。

An ultra high performance liquid chromatography （UPLC） method with mixed organic solvent extraction for simultaneously determining the phenylglyoxylic acid （PGA） and mandelic acid （MA） in urine samples was established, fifty urine samples of smokers and non-smokers were detected by the established method. The results showed that： 1） The extracted urine samples could be introduced directly after dilution with mobile phase, it only took 8 minutes to complete a UPLC analysis. 2） The concentration suitable for working curve of PGA and MA ranged from 0.425 to 21.500 μg/mL and from 1 to 100μg/mL, respectively; the correlation coefficients were above 0.9995. 3） The quantification limits and recoveries of PGA and MA were 0.013 and 0.077 μg/mL, 86.67%-96.33% and 88.50%-97.17%, respectively; and the intra-day and inter-day precisions were all less than 8% and 10%, respectively. 4） The contents of PGA and MA in smokers＇ urine samples were all higher than those in non-smokers＇ with significant differences （P〈0.05）. This method is simple, rapid and accurate for determining the contents of styrene metabolites in urine.