芙蓉李总多酚提取物对D-半乳糖所致亚急性衰老小鼠的抗氧化作用

Influence of Anti-oxidant Effective Fraction of Prunus Salicina on the D-Galactose Induced Sub-acute Aging Mice

目的观察芙蓉李总多酚提取物对D-半乳糖所致亚急性衰老小鼠的SOD、GSH-Px、MDA的影响。方法昆明种小鼠60只,♂,随机分为空白对照组、衰老模型组、阳性对照组、芙蓉李低剂量组、中剂量组、高剂量组。阳性对照组以维生素E软胶囊100 mg·kg-1·d-1灌胃给药,芙蓉李总多酚低、中、高剂量组分别以100,200,300 mg·kg-1·d-1灌胃给药,空白对照组与衰老模型组灌胃同体积的生理盐水,1次·d-1,连续灌胃42 d。给药的同时,衰老模型组与给药组小鼠,每日按注射剂量125 mg·kg-1·d-1颈背部皮下注射D-半乳糖,空白对照组小鼠皮下注射生理盐水。结果芙蓉李总多酚提取物给药组可以显著增强血清SOD活力,降低MDA含量,显著增强肝、脑组织SOD、GSH-Px活力,降低MDA含量,与衰老模型组比较,具有统计学差异(P<0.05)。结论不同剂量的芙蓉李总多酚提取物均能拮抗自由基损伤,具有抗氧化延缓衰老的作用,可能的机理是通过增加体内抗氧化酶的活性、减少过氧化脂质的生成,从而提高机体抗氧化能力。

OBJECTIVE To observe the total polyphenols extract of Furong Plum on the D-galactose induced subacute aging model SOD、GSH-Px activity and MDA content. METHODS 60 Kunming mice ,male,randomly divided into six groups,namely control group,model group, positive control group,low dose group,middle dose group,high dose group. positive control group were given vitamin E soft capsule in 100mg·kg-1？d-1. low dose group,middle dose group,high dose group were given the total polyphenols of Furong Plum in 100 mg？kg-1？d-1、200 mg？kg-1？d-1、300 mg？kg-1？d-1 respectively. control group and model group were given the same volume of physiological saline.once a day,for gavage 42 days.At the same time, model group and dose group mice were injected daily neck back D-galactose in 125 mg？kg-1？d-1.Control group injected with physiological saline. RESULTS The total polyphenols extract of Furong Plum can significantly enhance serum SOD activity and decrease MDA content. Also significantly enhance liver and brain tissue SOD、GSH-Px activity and decrease MDA content. Compared with model group, P 〈 0.05. CONCLUSION Different dosages of the total polyphenols extract of Furong Plum can antagonism free radical damage,has the function of antioxidation to delay senility. The possible mechanism is through the increase in the activity of antioxidant enzymes and reduce lipid peroxide formation, improve organism the oxidation resistance consequently.