枇杷叶总黄酮的纯化工艺及抗氧化活性研究

Study on Purification Technology and Antioxidant Activity of Total Flavonoid from Eriobotryae Folium

目的优选枇杷叶总黄酮分离纯化的工艺条件,考察枇杷叶总黄酮抗氧化活性。方法考察不同型号大孔树脂吸附洗脱能力,及大孔树脂、聚酰胺、硅胶对枇杷叶总黄酮的纯化能力,选择最佳分离纯化工艺,并采用ABTS自由基清除试验对黄酮提取物进行抗氧化活性评价。结果 D101树脂对枇杷叶总黄酮的吸附性能最好,最佳纯化工艺为D101树脂每1 g上样相当于1.5 g原药材样液,水、10%乙醇、40%乙醇、90%乙醇分别洗脱,洗脱流速为80 mL·h-1(12 BV·h-1),收集40%乙醇部分经D101大孔树脂同样条件二次洗脱,收集40%乙醇洗脱液得总黄酮提取物。提取物对ABTS自由基有较好的清除作用。结论 D101大孔树脂重复吸附是分离纯化枇杷叶总黄酮的理想方法。枇杷叶总黄酮具有良好的抗氧化活性。

OBJECTIVE To optimize the separate and purify technology of total flavonoid from Eriobotrya Japonica （Thunb.） Lindl., The antioxidant activity of total flavonoid was evaluated as well. METHODS Investigate the adsorption capacity and purification activity of resin, polyamide, silica gel. ABTS free radical-scavenging assay was used to test the antioxidant activity. RESULTS Resin D101 has the best activity to adsorb and purify total flavonoids, the optimal procedure is： 1g D101 resin, extract solution （1.5g powder of E. Japonica）, water, 10%, 40% and 90% ethanol gradient elute, adsorbing rate 80mL？h-1 （9BV？h-1）, 40% fraction is eluted and purified again by resin D101 with same process, then the 40% fraction was concentrated and dried to obtain total flavonoid. Total flavonoid showed a certain ABTS free radical-scavenging activity. CONCLUSION The repeated adsorption using resin D101 is the ideal method to separate and purify total flavonoid from E. Japonica.The total flavonoid of E. Japonica has a certain antioxidant activity.