颈痛舒贴片中芍药苷的体外透皮吸收研究

Study of Transdermal Absorption in Vitro of Paeoniflorin in Jingtong Shu Patch

目的研究颈痛舒贴片中芍药苷的体外透皮吸收特性,为其临床应用提供参考。方法采用改良Franz扩散池法,以离体小鼠皮肤为透皮屏障,生理盐水为接受介质,用高效液相色谱法进行测定,考察制剂中的芍药苷的累计透过量。结果芍药苷累计透过量Higuich方程为Q=2.667t1/2-4.204,r=0.859 3。结论芍药苷的累计透过量符合Higuich方程。

Objective To study the feature of paeoniflorin transdermal absorption in vitroin in Jingtong Shu patch ,so as to point out the direction for it’s clinical application. Methods Put to use the method of modified Franz diffusion pool , through excised mouse skin as transdermal barrier, with saline for accepting medium, use high performance liquid chromatography （HPLC） method for determination to investigate cumulative permeation amount of paeoniflorin in preparation. Results ：The Higuich equation of paeoniflorin cumulative permeation amount is Q=2.667t1/2-4.204,r=0.8593. Conclusion The cumulative permeation amount of paeoniflorin in accordance with Higuich equation.