UPLC-MS/MS测定延时类保健品中3种局麻药的含量

Quantitative Determination of Three Local Anesthetics in Delay Drugs by UPLC-MS/MS

目的 建立一种测定延时类保健品中三种局麻药含量的超高效液相-质谱联用方法。方法 采用Waters BEH C18（50 mm？2.1 mm,1.7 μm）色谱柱,流动相为含0.1%乙酸的0.02 mol.L-1乙酸铵溶液-乙腈（72：28）,流速为0.2 mL.min-1,柱温为35℃；质谱采用电喷雾离子源,多反应监测模式（正离子）。结果 三种局麻药在20 μg.L-1～200μg.L-1内线性关系良好,r值为0.9988～0.9998之间,平均回收率为98.2%～101.2%之间,分析方法的定量下限为0.5μg.L-1,检测限为0.1μg.L-1。结论 该方法分析速度快,灵敏度高,结果准确可靠,适用于延时类保健品中局麻药的测定,为打击保健品非法添加提供了有力的技术手段。

OBJECTIVE To establish a method for the quantitative determination of 3 local anesthetics in delay drugs by UPLC-MS/MS. METHODS Chromatography was carried on a Waters BEH C18 column（50 mm×2.1 mm, 1.7 μm） with the column temperature at 35 ℃, using 0.02 mol·L-1 ammonium acetate water solution（containing 0.1% acetic acid） -acetonitril （72：28） as mobile phase at a flow rate of 0.2 mL？min-1. Determination was performed by multiple reaction monitoring mode using electrospray ionization in positive ion mode. RESULTS Three chemical medicines had good linear relationships in 20 μg·L-1-200 μg·L-1 （r=0.9988-0.9998）. average recovery were 98.2%-101.2%.The limits of quantitation and detection were 0.5 μg·L-1 and 0.1 μg·L-1, respectively. CONCLUSION The method is rapid, sensitive and accurate, and could be an appropriate technique to detect the local anesthetics in delay drugs.