脂联素基因rs2241766、rs1501299多态性与结直肠癌发生风险研究

Association between adiponectin rs2241766, rs1501299 polymorphisms and the risk of colorectal cancer

目的探讨脂联素基因（ADIPOQ）rs2241766、rsl501299位点多态性与结直肠癌发生风险的关系，并分析2个位点与环境因素的交互作用在结直肠癌发生中的作用。方法采用1：1匹配的病例对照研究，应用自行设计的调查问卷对400例经组织病理学确诊的结直肠癌原发新发病例及400例同期体检者进行面对面调查，收集两组研究对象的一般情况、生活方式及饮食习惯等资料，应用聚合酶链反应一限制性片段长度多态性（PCR—RFLP）对两组研究对象ADIPOQrs2241766、rsl501299基因型进行检测。结果调整结直肠癌家族史、BMI、每日静坐时间、每周红肉摄入频次和经常饮茶等因素后，条件logistic回归分析结果表明，rs2241766TG＋GG携带者结直肠癌发生风险高于TT携带者（OR=1．354，95％CI：1．004～1．827），rs1501299GT＋TT携带者结直肠癌发生风险低于GG携带者（OR=O．680，95％CI：0．501-0．923）。广义多因子降维法分析结果显示，ADIPOQrs2241766、rsl501299与红肉摄入可能存在交互作用（P=0．001）。趋势x2分析显示，风险基因型携带个数与结直肠癌发生风险之间存在剂量一反应关系（X2=8．458，P=0．004）。结论ADIPOQrs2241766、rs1501299位点多态性可能与结直肠癌发生有关，且可能与红肉摄入存在交互作用，共同影响结直肠癌发生。

Objective To explore the association between adiponectin rs2241766,rs1501299 polymorphisms and gene-environment interaction and the risk of colorectal cancer （CRC）. Methods Four hundred CRC patients confirmed by histopathology and 400 healthy controls were recruited in this study. Cases and controls were matched on age and gender. A well-designed questionnaire was used to collect the information of demography, lifestyle and dietary habit on the 400 case-control pairs. Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was applied to detect the adiponectin rs2241766 and rs1501299 genotypes. Results Data from conditional logistic regression analysis showed that those carrying TG-t-GG genotype on rs2241766 having an increased risk compared to those that carrying TT genotype （OR = 1.354, 95% CI： 1.004-1.827）, and those that carrying GT ＋ TT genotype on rs 1501299 having an decreased risk when compared to those that carrying GG genotype （0R=0.680, 95% CI： 0.501-0.923） , after adjusted by factors as CRC family history, BMI, sedentary time, red meat consumption, and tea-drinking habit. Data from generalized multifactor dimensionality reduction showed that the gene-environment interaction among rs2241766, rs1501299 and red meat consumption on the risk of CRC might be significant（P=0.001 ）. A significant dosage effect with an increasing number of risk genotypes was observed as the risk of CRC increased （X2=8.458, P=0.004）. Conclusion Both adiponectin rs2241766 and rs1501299 were associated with CRC risk and the two SNPs might have worked together with red meat consumption in affecting the CRC risk.