四种梨皮中绿原酸的含量测定

Determination of Chlorogenic acid in four kinds of Pear skin by HPLC

摘要：目的采用反相高效液相色谱法测定四种梨皮中绿原酸的含量。方法色谱柱为AgilentTC—C19（4．6mm×250mm，5μm），流动相为乙腈一0．4％磷酸水溶液（7．5：92．5），检测波长为327nm，流速为1．0ml／min，柱温为30℃。结果线性关系良好，r=0．9999，回归方程为Y=33．241X一0．0359，线性范围为O．08—0．64Ixg，平均加样回收率为98．2％，RSD=1．8％（n=6）。结论方法简便、准确、重现性好，四种梨皮中绿原酸的含量差异较大，为中药梨皮的质量控制和评价提供了一定的科学依据。

Objective To develop a method using HPLC for the content determination of chlorogenic acid in four kinds of pear skin. Methods Aglient TC- C18 Column was used with acetonitrile -0.4% phosphoric solution（7.5：92.5） as the mobile phase, the detection wavelength at 327 nm, and flow rate was 1. OmL/min. The column temperature was maintained at 30~C. Re- suits The calibration curve was linear in the range of 0.08 - 0.64~g, r = 0. 9999. The linear equation is Y = 33. 241X - 0.0359. The average recovery was 98.2% , RSD = 1.8% （ n = 6）. Conclusion The method is simple, accurate and reproducible. The content of chlorogenic acid in four kinds of pear skin is different, which provides a scientific foundation for traditional Chinese medicine pear skin quality control and evaluation.