白术茯苓汤中用生白术或熟白术对湿困脾阻模型大鼠的影响

Effects of Baizhu Fuling Tang with Raw Atractylodes macrocephala or Processed A. macrocephala on Spleen Disorder due to Dampness Model Rats

目的:研究白术茯苓汤中用生白术或熟白术对湿困脾阻模型大鼠的影响。方法:复制大鼠湿困脾阻模型。50只SD大鼠分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、白术茯苓汤(生,8 g/kg)组、白术茯苓汤(熟,8 g/kg)组、平胃散(8 g/kg)组。测量大鼠体质量、腹围、腿围、平均进食量、饮水量,检测大鼠血清Na+-K+-ATP酶活性,神经降压素(NT)、P物质(SP)、血管活性肠肽(VIP)含量。结果:与正常对照组比较,模型组大鼠体质量减少,腹围、腿围增加,平均进食量、平均饮水量减少,差异有统计学意义(P<0.05);大鼠血清Na+-K+-ATP酶活性减弱,SP含量减少,NT、VIP含量增加,差异有统计学意义(P<0.05)。与模型组比较,白术茯苓汤(生)组大鼠体质量增加、腹围减少,平均进食量、平均饮水量增加,差异有统计学意义(P<0.05);大鼠血清Na+-K+-ATP酶活性增强,NT、VIP含量减少,差异有统计学意义(P<0.05)。结论:生白术之白术茯苓汤对湿困脾阻模型大鼠的保护作用较熟白术之白术茯苓汤为好,其作用机制可能是通过同时调节Na+-K+-ATP酶、NT及胃肠激素(SP、VIP)的分泌而达到治疗湿困脾阻的目的,白术茯苓汤中的生白术不可用熟白术替代。

OBJECTIVE： To study the effects of Baizhu fuling tang with raw Atractylodes macrocephala or processed A. macrocephala on control disorder due to dampness model rats. METHODS： Spleen disorder due to dampness model was established. 50 SD rats were divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, Baisu fuling tang group （raw A. macrocephala, 8 g/kg）, Baizhu fuling tang group （processed A. macrocephala, 8 g/kg） and Pingwei powder group （8 g/kg）. Body weight, abdominal perimeter , leg perimeter, average food-intake and the amount of drinking water were all determined; the activity of Na＋-K＋-ATP enzyme was detected; the contents of NT, SP and VIP were measured. RE- SULTS： Compared with normal control group, body weight of rats decreased significantly in model group, while abdominal perim- eter and leg perimeter increased significantly; average food-intake and the amount of drinking water decreased significantly; there was statistical significance（P〈0.05） ; the activity of Na＋-K＋-ATP enzyme and SP content in serum decreased significantly, while the contents of NT and VIP increased significantly; there was statistical significance（P〈0.05）. Compared with model group, body weight, average food-intake and the amount of drinking water of rats in Baizhu fuling tang group （raw A. macrocephala） increased significantly, while abdominal perimeter decreased significantly; there was statistical significance （P〈0.05）. The activity of Na＋-K＋-ATPase in serum increased significantly, while the contents of NT and VIP decreased significantly; there was statistical significance （P〈0.05）. CONCLUSIONS： Baizhu fuling tang with raw A. macrocephala is better than that with processed A. macrocephala in the treatment of spleen disorder due to dampness. Its mechanism may be associated with the secretion regulation of Na＋-K＋-ATP enzyme,NT, SP and VIE Raw A. macrocephala in Baisu ruling tang can not be replaced by processed A. macrocephala.