摘要
目的:建立测定荜茇根中胡椒碱含量的方法。方法:采用高效液相色谱法。色谱柱为Inertsil ODS-3(250mm×4.6mm,5μm),流动相为甲醇-水(77:23,V/V),检测波长为343nm,流速为1.0ml/min,柱温为30℃。结果:胡椒碱的质量浓度在0.0008~0.0240mg/ml范围内与其峰面积积分值呈良好的线性关系(r=0.9999);精密度、稳定性、重复性试验的RSD〈2%;平均加样回收率为100.85%,RSD=0.82%(n=6)。结论:该方法简便、快速、准确、重复性好,可用于荜茇药材的质量控制。
OBJECTIVE: To establish a method for simultaneous determination of piperine in the roots of Piper longum. METHODS: HPLC method was adopted. The determination was performed on Inertsil ODS-3 (250 mm ×4.6 mm, 5 μm) column with mobile phase consisted of methanol-water (77 : 23, V/V) at the flow rate of 1.0 ml/min. The detection wavelength was set at 343 nm, and column temperature was 30 ℃. RESULTS: The linear range of piperine were 0.000 8-0.024 0 mg/ml(r=0.999 9); RSDs of precision, stability and reproducibility tests were lower than 2% ; average recovery was 100.85% (RSD=0.82%, n=6). CONCLUSIONS: The method is simple, rapid, accurate and reproducible, and can be used for quality control for the roots of P longum.
出处
《中国药房》
CAS
CSCD
2014年第7期633-635,共3页
China Pharmacy
基金
国家自然科学基金资助项目(No.81260670)
关键词
高效液相色谱法
荜茇根
胡椒碱
含量测定
HPLC
The roots of Piper longum
Piperine
Content determination