HPLC法测定荜茇根中胡椒碱的含量

Content Determination of Piperine in the Roots of Piper longum by HPLC

目的：建立测定荜茇根中胡椒碱含量的方法。方法：采用高效液相色谱法。色谱柱为Inertsil ODS-3（250mm×4．6mm，5μm），流动相为甲醇-水（77：23，V／V），检测波长为343nm，流速为1．0ml／min，柱温为30℃。结果：胡椒碱的质量浓度在0．0008～0．0240mg／ml范围内与其峰面积积分值呈良好的线性关系（r=0．9999）；精密度、稳定性、重复性试验的RSD〈2％；平均加样回收率为100．85％，RSD=0．82％（n=6）。结论：该方法简便、快速、准确、重复性好，可用于荜茇药材的质量控制。

OBJECTIVE： To establish a method for simultaneous determination of piperine in the roots of Piper longum. METHODS： HPLC method was adopted. The determination was performed on Inertsil ODS-3 （250 mm ×4.6 mm, 5 μm） column with mobile phase consisted of methanol-water （77 ： 23, V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 343 nm, and column temperature was 30 ℃. RESULTS： The linear range of piperine were 0.000 8-0.024 0 mg/ml（r=0.999 9）; RSDs of precision, stability and reproducibility tests were lower than 2% ; average recovery was 100.85% （RSD=0.82%, n=6）. CONCLUSIONS： The method is simple, rapid, accurate and reproducible, and can be used for quality control for the roots of P longum.