伪狂犬病病毒立即早期180基因的克隆及序列分析

Cloning and Sequence Analysis of the IE180 Gene of Pseudorabies Virus

为了研究伪狂犬病病毒（pseudorabies virus，PRV）立即早期180基因（immediate early 180，IE180）及其功能，试验采用设计的特异性引物通过PCR法对IE180基因进行扩增，并成功构建了IE180克隆载体，经序列测定后，用DNAStar软件对该序列和已发表的7个PRV参考株的IE180基因核苷酸序列及推导的氨基酸序列进行比对分析。结果表明，HB-98株IE180基因编码区大小为4425 bp，编码1474个氨基酸，与GenBank中登录的参考株的IE180基因核苷酸同源性为98.4%～99.1%,氨基酸同源性为97.8%～98.4%。系统进化树分析结果表明，该毒株与毒株TNL（登录号：AF352564.2）的亲缘关系较近。

To study immediate early 180 （IE180） gene of pseudorabies virus （PRV） and its function，IE180 gene was amplified by PCR method with designed specific primers, and then IE180 cloning vector was successfully constructed. After sequence determination, IE 180 gene sequence and amino acid sequence were compared and analyzed by using DNAStar software among seven published PRV reference strains. The results showed that the CDS region of IE 180 gene consisted of 4425 bp, coded 1474 amino acids，the gene and amino acid sequence homologies between HB-98 strain and reference strains were 98.4% to 99.1% and 97.8% to 98.4%. Phylogenetic tree analysis demonstrated that HB-98 strain was genetically closely related to TNL strain（AF352564.2）.