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山羊可溶型干细胞因子mRNA在不同毛色皮肤组织中的表达水平

Expression Level of Soluble Stem Cell Factor mRNA in Skin with Different Coat Color
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摘要 为探讨可溶性干细胞因子(stem cell factor,SCF)基因表达水平与山羊毛色间的相关性,本试验利用实时荧光定量PCR(QRT-PCR)技术研究了SCF基因mRNA在黑色和白色山羊皮肤中的表达量,并对结果进行统计分析。经内参基因校正后,黑色山羊皮肤中可溶型SCF的相对表达量是白色山羊相对表达量的0.27倍(P>0.05)。可溶型SCF mRNA表达量可能与山羊毛色表型不存在相关性。 To investigate the relationship between the expression level of soluble stem cell factor （SCF） and goat coat color. The expression level of soluble SCF in goat skins with black and white coat color was analyzed with QRT-PCR technique and statistics software. The relative expression level of soluble SCF in skin with black coat color was 0.27 times higher than that with white coat color normalization with GAPDH gene. It suggested that the expression level of SCF gene might not be related to coat color in goat.
作者 张军杰 周荣艳 锡建中 李祥龙 李兰会
机构地区 河北农业大学动物科技学院 河北省牛羊胚胎工程技术中心
出处 《中国畜牧兽医》 CAS 北大核心 2014年第1期57-61,共5页 China Animal Husbandry & Veterinary Medicine
基金 河北省自然科学基金项目(C2012204009) 河北省教育厅项目(Q2012074)
关键词 干细胞因子 可溶型 山羊 毛色 皮肤 stem cell factor soluble goat goat color skin
分类号 Q786 [生物学—分子生物学]
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参考文献11

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中国畜牧兽医
2014年 第1期

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