兰州地区部分奶牛场子宫内膜炎病原菌分离鉴定及抗生素耐药性研究

Isolation and Identification of Endometritis Pathogens in Dairy Farms Located in Lanzhou City and its Antibiotic Resistance Analysis

为了探明兰州地区奶牛子宫内膜炎病原菌区系分布及抗生素耐药情况，指导临床合理用药。笔者对从兰州地区3个规模化奶牛场采集的47份奶牛子宫内膜炎样品进行了细菌分离鉴定，同时对分离鉴定出的4种主要病原菌采用纸片扩散法（K-B法）进行了抗生素耐药性检测。病原菌区系分布结果表明，引起兰州地区奶牛子宫内膜炎的病原菌主要为化脓隐秘杆菌（37.1%）、大肠杆菌（24.2%）、屎肠球菌（22.6%）和无乳链球菌（16.1%）。抗生素耐药性结果表明，4种主要病原菌对大部分抗生素均产生了不同程度的耐药性，尤其是对青霉素、复方新诺明、奥复星和杆菌肽耐药性最为严重，其耐药率为60%～100%。对4种病原菌均较敏感的药物主要有头孢他啶、先锋霉素V和氟苯尼考，其敏感度达100%。

This study was planned to investigate the distribution and antibiotic resistance of pathogens involve in cow endometritis，which will be helpful to guide clinical rational drug use.All 47 cow endometritis samples collected from three large-scale farms located in Lanzhou were used to isolate and identify pathogens. Besides，K-B paper disk method was performed to measure the antibiotic resistance of the four isolates. The results demonstrated that Arcanobacterium pyogenes （37.1%），Escherichia coli （24.2%），Enterococcus faecium （22.6%） and Streptococcus agalactiae （16.1%） were the predominent agent causing cow endometritis in Lanzhou.In addition，the four pathogens were resistant to penicillin，sulfamethoxazole compound，ofoxacin and bacitracin，and the drug resistance rates reached 60% to 100%. However，These pathogens were sensitive to ceftazidime，cephalothin V and florfenicol，and the antibiotic sensitive rates were 100%.